Simon Scheuring, Professor of Physiology and Biophysics in Anesthesiology, Weill Cornell Medical College, will give a talk on "High-Speed Atomic Force Microscopy Single-Molecule Structural Biology Uncovers Rare and Transient Conformational States"

Zoom link: https://gc-cuny.zoom.us/j/91032629703. Meeting ID:  910 3262 9703. Passcode: ASRC+CCNY

ABSTRACT

High-speed atomic force microscopy (HS-AFM) is a powerful technique that provides dynamic movies of biomolecules at work. First, I will briefly review our recent developments to break temporal limitations to characterize molecular dynamics by developing HS-AFM line scanning (HS-AFM-LS) and HS-AFM height spectroscopy (HS-AFM-HS), and resolution limitations by developing Localization AFM (LAFM). Then, I will detail how we used HS-AFM to analyse membrane-embedded TRPV3 channels at the single-molecule level, and discovered a previously unobserved, transient, and reversible pentameric state.Then, I will report about our most recent efforts to introduce single-molecule structural biology solving multiple structures of a single molecule along its functional timeline. Finally, if time permits, I will show some of our most recent developments that may make HS-AFM faster and more sensitive in the near future.