Melanie Cocco, Associate Professor, the Dept. of Pharmaceutical Sciences, and the Dept. of Molecular Biology & Biochemistry, at the University of California, Irvine, will present a talk titled, "Protein Dynamics Determined from NMR Hydrogen Exchange Measurements and Hemoglobin Stability with Voxelotor."

ABSTRACT

NMR spectroscopy provides a measure of protein dynamics in solution that is useful in understanding the relative stabilities of mutants, drug-bound states, or homologous proteins.  Hydrogen-deuterium exchange reactions occur during a timescale specific to protein folding events and can provide details on local unfolding and/or global stability.  Here we present three studies of hydrogen exchange in DNA-binding protein domains (DBDs).  The cytidine repressor (CytR) is a bacterial sensor that regulates the production of enzymes needed for nucleic acid production.  CytR has been identified as a target in the treatment of cholera and urinary tract infections.  We compare the structure and stability of the CytR protein to the lactose repressor (LacR).  Hydrogen exchange for another system, the DBD of the p53 transcription regulator reveals how the structure of this protein can be stabilized by rescue mutations that restore function of this important anti-cancer protein.  A third system, a thermally stable archaeal bypass polymerase Dbh is shown by hydrogen exchange to maintain structural features in proximity to the catalytic domain at high temperatures. 

Hemoglobin is the last system to be discussed.  Voxelotor (aka Oxbryta) is a new drug developed to treat sickle cell disease by binding the hemoglobin tetramer and maintaining the protein in a stable soluble conformation.  We examine the effect of this drug on the natural degradation of hemoglobin in solution.

Zoom link: https://gc-cuny.zoom.us/j/96677631144. Meeting ID:  966 776 31144. Passcode: asrc-ccny