Christiane Riedel, Professor, Department of Biology, Ecole Normale Superieure de Lyon, Lyon, France, will be giving a talk by Zoom titled: "Unravelling virus architecture and entry employing cryo electron tomography"

Zoom link: https://gc-cuny.zoom.us/j/91637964386

Meeting ID:  916 3796 4386. Passcode: asrc+ccny

ABSTRACT

Cryo electron tomography (CET) is a powerful technique to elucidate virus architecture and virus replication cycles. It has opened up the possibility to study structures of complex viruses and virus cell interactions in a frozen, hydrated state, thereby preserving biological features in near native conditions. Employing this technical approach, we determined the structure of the rabies virus ribonucleoprotein in intact virus particles to 15Å resolution and demonstrated morphological differences to the related vesicular stomatitis virus. Employing murine leukaemia virus as a model system, we elucidated structural changes of Env during interaction with host cells, demonstrating an elongation of the protein when in contact with the host cell. Lastly, the experimental systems needed to study virus host interactions by CET will be discussed based on our work with bovine viral diarrhoea virus.