Ph.D.  1994  Yale University

M. Phil  1989 Yale University

B. S.  1987  University of Cincinnati
 

Chem 440/A4000: Journey to the Center of the Cell

Chem B9800: Biochemistry Seminar

Chem 87901: Molecular Biophysics (at Graduate Center)

Biochemistry/Structural Biology/Biophysics

The faithful transmission of genetic information is an important biological imperative. To carry out this function, organisms have evolved processes to replicate their genomes and defend them from attack. We study two important mechanisms associated with these processes, DNA replication and nucleotide excision repair. My group applies X-ray crystallography, supplemented with electron microscopy, to understand these long-standing problems in DNA biology. We also use biochemical studies to inform these approaches and follow up on the resulting insights.

Case BC, Hartley S, Osuga M, Jeruzalmi D, Hingorani MM. The ATPase mechanism of UvrA2 reveals the distinct roles of proximal and distal ATPase sites in nucleotide excision repair. Nucleic Acids Res. 2019 May 7;47(8):4136-4152. doi: 10.1093/nar/gkz180. PubMed PMID: 30892613; PubMed Central PMCID: PMC6486640.

Chase J, Catalano A, Noble AJ, Eng ET, Olinares PD, Molloy K, Pakotiprapha D, Samuels M, Chait B, des Georges A, Jeruzalmi D. Mechanisms of opening and closing of the bacterial replicative helicase. Elife. 2018 Dec 24;7. pii: e41140. doi:10.7554/eLife.41140. PubMed PMID: 30582519; PubMed Central PMCID: PMC6391071.

Noble AJ, Dandey VP, Wei H, Brasch J, Chase J, Acharya P, Tan YZ, Zhang Z, Kim LY, Scapin G, Rapp M, Eng ET, Rice WJ, Cheng A, Negro CJ, Shapiro L, Kwong PD, Jeruzalmi D, des Georges A, Potter CS, Carragher B. Routine single particle CryoEM sample and grid characterization by tomography. Elife. 2018 May 29;7. pii:e34257. doi: 10.7554/eLife.34257. PubMed PMID: 29809143; PubMed Central PMCID: PMC5999397.

Kraithong T, Channgam K, Itsathitphaisarn O, Tiensuwan M, Jeruzalmi D, Pakotiprapha D. Movement of the β-hairpin in the third zinc-binding module of UvrA is required for DNA damage recognition. DNA Repair (Amst). 2017 Mar;51:60-69. doi: 10.1016/j.dnarep.2017.02.003. Epub 2017 Feb 7. PubMed PMID: 28209516.

Orlova N, Gerding M, Ivashkiv O, Olinares PDB, Chait BT, Waldor MK, Jeruzalmi D. The replication initiator of the cholera pathogen's second chromosome shows structural similarity to plasmid initiators. Nucleic Acids Res. 2017 Apr 20;45(7):3724-3737. doi: 10.1093/nar/gkw1288. PubMed PMID: 28031373; PubMed Central PMCID: PMC5397143.

Pakotiprapha D, Jeruzalmi D. Small-angle X-ray scattering reveals architecture and A₂B₂ stoichiometry of the UvrA-UvrB DNA damage sensor. Proteins. 2013 Jan;81(1):132-9. doi: 10.1002/prot.24170. Epub 2012 Sep 28. PubMed PMID: 22933319.

Pakotiprapha D, Samuels M, Shen K, Hu JH, Jeruzalmi D. Structure and mechanism of the UvrA-UvrB DNA damage sensor. Nat Struct Mol Biol. 2012 Feb 5;19(3):291-8.  doi: 10.1038/nsmb.2240. PubMed PMID: 22307053.