LSM 800

Location and contact information

  • Location: CCNY, Marshak 525
  • Facility Manager: Jorge Morales, Ph.D.
  • Phone:  212 650 8591
  • Hours: Open 24/7 to qualified users.
  • Scheduling calendar: click here

Equipment Specifications

  • Objectives: 10x,20x,40x-oil,63x-oil,100x
  • Lasers: 405, 488, 561, 649
  • Detectors: Airyscan 32 Channel, GaAsP x 2, TPMT (DIC, BF)
  • Filters: SP 470, SP 545, SP620, LBF640, LP575, LP655, SP620
  • Live cell imaging: Humidity, Temperature, C02 control.
  • Max. Resolution: 250nm. Airyscan enhanced 150nm
  • Software: ZEN Blue 2017 2.3

Fees

Commercial users - $31/h
Federal users - $20/h

Long-running experiments qualify for a 50% discount.  See Add. I in the facility usage rules for more info.

In addition federal grant holders qualify for a fee cap of $1500/month for all usage (combined) of LSM 800, LSM 880, and Nikon TiE.

Images

Lab View 1

Background Information

LSM 800 is a continuation of efforts to innovate and break barriers in traditional optical imaging. The use of highly sensitive detectors and new imaging hardware allows researchers to achieve more resolution thus see more details scarifying neither samples nor good imaging practices. The microscope has been configured to serve the needs of CCNY community focusing particularly on live-cell imaging. High sensitivity detectors and airyscan technology combined with hand picked lenses will put another outstanding tool in hands of researchers working on projects at CCNY Core Facilities.

The technology worth highlighting again is the Airyscan which solves conundrum between resolution and light efficiency by imaging the complete airy disk onto a concentrically arranged hexagonal detector array. It consists of 32 single detector elements, all of which act like sub airy unit pinholes. The confocal pinhole itself remains open and doesn’t block light – thus all photons of the whole airy disk are collected.

The signals from all detector elements are then reassigned to their correct position, producing an image with increased signal-to-noise ratio and resolution. Unlike other superresolution techniques, Airyscan capitalizes on the scanning and optical sectioning capabilities of a confocal. Thus Airyscan even works with thicker samples such as tissue sections or whole animal mounts that need a higher penetration depth. (source Zeiss.com)

Rules and Regulations

Rules governing the usage of the facility.

Documents

Introduction to Ziess LSM 800

Guide to AiryScan (PDF)

Additional Resources:

Dyes: https://www.chroma.com/spectra-viewer

LSM800 Airyscan: https://www.youtube.com/watch?v=--9F1Xa2I_A

Fluorescence: http://www.thermofisher.com/us/en/home/support/tutorials.html#vid1

Last Updated: 09/19/2023 13:13